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In this panel of four images, cells are stained with an immunostain against substance P and labelled with an in situ probe for c-fos. The image demonstrates the use of polarization epifluorescence microscopy for visualizing silver grains lying over heavily stained cells. Specimen courtesy of B. Kosofsky, Harvard University.
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The top two panels demonstrate automatic detection and counting of a 35S oligoprobe for oxytocin mRNA in human hypothalamus (specimen courtesy of J. Palacios, Barcelona).
The bottom two panels demonstrate automatic detection and counting in a thionin-stained mouse brain section labelled with 125I estrogen. Specimen courtesy of N. Maclusky, University of Toronto.
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