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MCID & AIS Application Guides



Software Version: MCID Elite Workstation 6.0

Application Guides

Gel Analysis
Acquiring a density profile · Automatic baseline subtraction · Manual and automatic quantitation of peaks/bands · Smiling correction · Establishing density and/or molecular weight calibrations · Direct band analysis · Creating custom data reports
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Grain Counting
Overview of basic procedure · Establishing target criteria · Collecting data · Editing the scan · Maximizing grain visibility · Re-directed sampling with two images · Counting clumps of grains
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Quantitative Autoradiography
Overview of basic procedure · Flat field correction · Establishing a density calibration · Labeling data · Combining autoradiography and histology · Summarizing data
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Receptor Binding
Overview of basic procedure · Aligning images · Assigning nonspecific binding values · Combining autoradiography and histology · Creating a specific binding image
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Stereology
2D Frames measures · 3D Probes measures · 3D Disectors · 3D Fractionator · Section Types · Stereo sampling grid · Establishing target detection criteria · Collecting data · Automatic sampling across an image set · Automated optical fractionator · Tutorial lessons
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