
Software Version: MCID Elite 6.0
Cameras Setup Guides
CoolSNAP Color Camera
Connecting components · Installing the driver · Installing in AIS · Acquiring images · Adjusting the camera response · Maintenance and troubleshooting |
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Dage MTI Cameras
For Dage MTI CCD72, 72S, 300RC and 300TRC · Connecting components · Installing in AIS · Acquiring images · Adjusting the camera response |
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DVC Digital Cameras
Connecting components · Installing in MCID Elite · Acquiring images · Adjusting the camera response · Maintenance and troubleshooting |
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Hamamatsu Digital Cameras
For C4880 and C4742 · Connecting components · Installing in MCID Elite · Acquiring images · Adjusting the camera response |
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PVCAM Digital Cameras
For CoolSNAP™ mono, CoolSNAPcf™ mono, CoolSNAPfx™, CoolSNAPHQ™, SenSys®, Quantix® · Connecting components · Installing the driver · Installing in AIS · Acquiring images · Adjusting the camera response · Maintenance and troubleshooting · Making color images with filters |
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Sony DXC Color Cameras
For Sony® DXC 930/960, 950/970, 390 · Connecting components · Installing in AIS · Acquiring images · Adjusting the camera response |
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Spot RT Digital Cameras
For SPOT RT™ Mono, Color and Slider · Installing the camera driver · Connecting components · Installing in AIS · Acquiring images · Adjusting the camera response |
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Xillix MicroImager
Connecting components · Installing in MCID Elite · Acquiring images · Adjusting the camera response · Maintenance and troubleshooting |
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Application Guides
3D Reconstruction
The M3D Module · Creating an image stack · Aligning, unwarping and normalizing images · Defining structures · Creating a 3D Dataset · Contour rendering · Surface rendering · Density rendering · Structure rendering · Revealing internal structures · Applying a cutting plane to a rendering · Recording and playing cine loops · Making 3D measurements · Tutorial lessons |
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Dynamic Fluorescence
Single wavelength and ratiometric imaging · Wavelength/filter selection · Ratio timing setup · Calibrating ratio experiments · Camera setup · Acquring images · Displaying wavelength and ratio images · Gathering data · Graphing ion kinetics · Applying deconvolution algorithms · Aligning serial images · Recording and playing cine loops · Saving and retrieving experiments · Fura-2 external calibration procedure |
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Gel Analysis
Acquiring a density profile · Automatic baseline subtraction · Manual and automatic quantitation of peaks/bands · Smiling correction · Establishing density and/or molecular weight calibrations · Direct band analysis · Creating custom data reports |
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Grain Counting
Overview of basic procedure · Establishing target criteria · Collecting data · Editing the scan · Maximizing grain visibility · Re-directed sampling with two images · Counting clumps of grains
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Quantitative Autoradiography
Overview of basic procedure · Flat field correction · Establishing a density calibration · Labeling data · Combining autoradiography and histology · Summarizing data |
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Receptor Binding
Overview of basic procedure · Aligning images · Assigning nonspecific binding values · Combining autoradiography and histology · Creating a specific binding image |
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Stereology
2D Frames measures · 3D Probes measures · 3D Disectors · 3D Fractionator · Section Types · Stereo sampling grid · Establishing target detection criteria · Collecting data · Automatic sampling across an image set · Automated optical fractionator · Tutorial lessons |
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