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Details
MCID™ Core is a modular applications based acquisition and image analysis software and is easily optimized and configured for single applications based on specific user requirements.
Display
MCID™ uses a single monitor, and displays the aquired image and the analysis data in the same window. Therefore, we recommend a high resolution display, typically 1600x1200 pixels x 24 bits (16 million colors). MCID™ Core can also work with dual screens using Windows XP dual monitor support. For dual monitor support we recommend using NVIDIA graphics card with at least 256MB RAM running at a resolution of 1600x1200 per screen.
Multiple Image Channels
MCID™ offers multiple, independent image channels (16 in total). Each channel can contain a discrete, calibrated image. Use the multiple image channels to align stained sections and autoradiographs, or to display images acquired at different fluorescence wavelengths. Each channel can have varying size images and are not limited by image size.
Image input / saving functions MCID™ accepts and exports images in a variety of formats, including monochrome and color TIFF, MCID™, and in the native formats of many cameras and scanners. For other file formats, a general-purpose image format editor is supplied as standard. You can easily create your own image file format specifications, and integrate them within MCID™.
Image acquisition
MCID™ supports a number of Digital CCD cameras from some of the leading manufactures including the CoolSNAP range from Photometrics and now also the Qimaging range of Firewire cameras. With the addition of a compatible PCI image capture board MCID™ also supports a number of older analog video cameras as well.
Image input / saving functions MCID™ accepts and exports images in a variety of formats, including monochrome and color TIFF, MCID™, and in the native formats of many cameras and scanners. For other file formats, a general-purpose image format editor is supplied as standard. You can easily create your own image file format specifications, and integrate them within MCID™.
Additional hardware MCID™ supports motorized microscope stages and Z-axis drives from Prior, Ludl (LEP) & Märzhäuser), as well as shutters and filter wheels from Prior, Ludl (LEP) & Sutter, for controlling illumination and excitation wavelengths. MCID™ also supports the OASIS 4i controller which allows control of most microscope stages using a standard single properietry PCI card. In addition to Z drives MCID™ can also control the PIFOC motorised objective motor.
Motorised stages give MCID™ the ability to create high resolution tiled images automatically using the Automated Image Stitching module. With an motorised stage MCID™ can also be used to as a Optical Fractionator using the optional stereology module.
Calibration Powerful calibration options allow you to calibrate images to external density and spatial standards. Nonlinear calibrations (e.g. molecular weight) fully supported.
Create calibration curves with best fit curve type options, special calibration functions for Glucose Untilisation, Protein Synthesis & Regional Blood Flow.
Create microscope nosepiece files to hold your objective calibrations.
Data gathering & export Powerful and flexible set of data sampling tools, including automatic tools. Data is saved to an MCID™ log file which contains scan shapes information and subject, section and region labelling, data can then be exported via the clipboard or in Excel, CSV, ASCII & Lotus formats.
Image fusion - Collect any number of discrete fluor images.
Fusion functions create 24-bit color images. Functions range from simple addition to weighted addition with layer-specific transparency.
Image processing and editing -
Apply filters, logical operators, image arithmetic. Edit images to remove artifacts, or to create image montages.
Z Focus & Deconvolution - MCID™ includes both No-neighbour and Nearest-neighbour deconvolution algorithms. In addition MCID™ can also perform Z focusing using a motorised stage, you can set MCID™ to either autofocus or to create a single in-focus image from multiple z stacked images.
Image Registration - Perform image registration on saved images or on live images using our shadow align functionality.
Easy report generation
Report generator creates summary reports including numerical data, profile graphs, and image.
Print reports with one command.
Gel/Blot analysis Nonlinear calibrations in X and Y dimensions.
1D mode.
1D mode for TLC.
Corrections for bent or stretched lanes, smiling.
Vertical, horizontal, rotatable, and user-traced lane definition tools.
Display and analyze multiple lanes, simultaneously, or one at a time.
Manual or automatic peak detection. Manual or automatic baseline correction.
Powerful profile tools
Vertical, horizontal, rotatable, and user-traced lane definition tools.
Display and analyze multiple profiles, simultaneously, or one at a time.
Zoom in on any portion of the profile for high-resolution display.
Fully automatic target detection For grain counts, and other feature distribution analyses, fully automated target detection is supported. Multiple density thresholds can be combined with logical criteria (e.g. area) to separate targets from background.
Sampling tools
Sampling tools are probes used to gather data from images. Tools can be sized and rotated by the user.
Sample Tools
rectangle
circle
trace
automatic
template
paint
polygon
whole field
distance
angle
pixel report
arrays
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