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MCID Core

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MCID Core Digital Imaging Software

 

MCID Core SoftwareMCID™ Core is a complete imaging software solution. MCID™ Core can be optimized and configured for single applications based on specific user requirements. MCID™ will accept image files in many formats and is an ideal host for phosphor imagers, laser scanners, scanning densitometers and similar devices. Camera support is via many of the compatible digital cameras such as the CoolSNAP or Qimaging range. MCID™ Core is available as an off the shelf software solution or as part of a number of software/hardware "packages" optimized for specific applications.

 

 

 

Digital Densitometry

 

mcid autoradiography softwareThe MCID™ Densitometry System has served thousands of scientists for over 15 years. The system includes calibrations and corrections which are easily applied to yield precise quantitative and semi-quantitative data. The MCID™ Densitometry System can be used with a wide variety of cameras, scanners and phosphor images.

Fluorescence Microscopy

 

mcid fluorescence softwareThe MCID™ Fluorescence System captures, analyzes and manipulates fluorescence images with ease. Measure label intensity, combine discrete fluors, use for co-localization and clarify using digital deconvolution. The MCID™ Fluorescence System supports video and digital cameras, color or monochrome, standard, chilled or cooled. Cryogenic cameras are supported for the most demanding applications.

Gel / Blot Analysis

 

mcid gel softwareMCID™ acquires, annotates, archives, prints and quantifies images of your gels and blots. Use MCID™ as a simple archival system or take advantage of the extensive list of analysis functions. Digitize your specimens with any input device, including scanners, and video or digital cameras. 

Automated Image Stitching

 

automated image stitchingThe MCID™ Image Stitching System automatically merges multiple fields of view into a single, large image. The image is seamless, sharply focused and has high resolution. Create spectacular, high quality images. Ideal for visualizing details within a larger biological framework and for high quality publication.

Stereology

 

mcid stereologyThe MCID™ Stereology module applies commonly used stereological measures including volume, surface area, length and counts to your images. Integrate stereology within the broad function set of your MCID™ image analyzer. Add a motorised stage controller to enable the optical fractionator wizard. Microscope stages are available to retrofit to any make and model of microscope.

Grain & Cell Counts

 

cell and silver grain countingMCID™'s thresholding facility allows the user to threshold valid targets and apply morphological criteria, pass or reject objects for analysis based on many criteria such as size and shape. The threshold can than be applied to parts of the image or all of image as the user sees fit. Coupling thresholding and our AutoScan A & B tools users can remove the need to manually trace regions of interest.

 

 

 

MCID™ Offers a powerful multipurpose image suite that would be a welcomed edition to any facility. That's why MCID™ has over 3000+ users worldwide from many of the worlds leading Pharmaceutical Companies, Universities and Research Facilities.

Details

MCID™ Core is a modular applications based acquisition and image analysis software and is easily optimized and configured for single applications based on specific user requirements.

Applications available include: 

  • Autoradiography, Receptor Binding, Glucose Untilization & Regional Blood Flow
  • Grain & Cell Counting
  • Fluorescence Microscopy
  • Colocalisation
  • Gel / Blot Analysis
  • Automated Image Stitching
  • Stereology - Optical Fractionator

 

MCID Dual Screen Support

Display

 

MCID™ uses a single monitor, and displays the aquired image and the analysis data in the same window. Therefore, we recommend a high resolution display, typically 1600x1200 pixels x 24 bits (16 million colors). MCID™ Core can also work with dual screens using Windows XP dual monitor support. For dual monitor support we recommend using NVIDIA graphics card with at least 256MB RAM running at a resolution of 1600x1200 per screen.

 


 

Multiple Image Channels


MCID™ offers multiple, independent image channels (16 in total). Each channel can contain a discrete, calibrated image. Use the multiple image channels to align stained sections and autoradiographs, or to display images acquired at different fluorescence wavelengths. Each channel can have varying size images and are not limited by image size.
MCID Multiple Channels
 
MCID Core TPL Editor

Image input / saving functions


MCID™ accepts and exports images in a variety of formats, including monochrome and color TIFF, MCID™, and in the native formats of many cameras and scanners. For other file formats, a general-purpose image format editor is supplied as standard. You can easily create your own image file format specifications, and integrate them within MCID™.
       

Image acquisition

 

MCID™ supports a number of Digital CCD cameras from some of the leading manufactures including the CoolSNAP range from Photometrics and now also the Qimaging range of Firewire cameras. With the addition of a compatible PCI image capture board MCID™ also supports a number of older analog video cameras as well.
MCID camera example


MCID Core TPL Editor

Image input / saving functions


MCID™ accepts and exports images in a variety of formats, including monochrome and color TIFF, MCID™, and in the native formats of many cameras and scanners. For other file formats, a general-purpose image format editor is supplied as standard. You can easily create your own image file format specifications, and integrate them within MCID™.

           

Additional hardware


MCID™ supports motorized microscope stages and Z-axis drives from Prior, Ludl (LEP) & Märzhäuser), as well as shutters and filter wheels from Prior, Ludl (LEP) & Sutter, for controlling illumination and excitation wavelengths. MCID™ also supports the OASIS 4i controller which allows control of most microscope stages using a standard single properietry PCI card. In addition to Z drives MCID™ can also control the PIFOC motorised objective motor.

Motorised stages give MCID™ the ability to create high resolution tiled images automatically using the Automated Image Stitching module. With an motorised stage MCID™ can also be used to as a Optical Fractionator using the optional stereology module.
MCID Multiple Channels
  
         
Calibration screen

Calibration


Powerful calibration options allow you to calibrate images to external density and spatial standards. Nonlinear calibrations (e.g. molecular weight) fully supported.

Create calibration curves with best fit curve type options, special calibration functions for Glucose Untilisation, Protein Synthesis & Regional Blood Flow.

Create microscope nosepiece files to hold your objective calibrations.

 
          

Data gathering & export


Powerful and flexible set of data sampling tools, including automatic tools. Data is saved to an MCID™ log file which contains scan shapes information and subject, section and region labelling, data can then be exported via the clipboard or in Excel, CSV, ASCII & Lotus formats.

MCID Data Export

           
Image Fusion

Deconvolution example

Powerful Image Functions


Manipulate contrast, pseudocolor coding, nonlinear image mapping.

Image fusion - Collect any number of discrete fluor images. Fusion functions create 24-bit color images. Functions range from simple addition to weighted addition with layer-specific transparency.

Image processing and editing - Apply filters, logical operators, image arithmetic. Edit images to remove artifacts, or to create image montages.

Z Focus & Deconvolution - MCID™ includes both No-neighbour and Nearest-neighbour deconvolution algorithms. In addition MCID™ can also perform Z focusing using a motorised stage, you can set MCID™ to either autofocus or to create a single in-focus image from multiple z stacked images.

Image Registration - Perform image registration on saved images or on live images using our shadow align functionality.



           

Easy report generation

 

Report generator creates summary reports including numerical data, profile graphs, and image.

Print reports with one command.


Data report option

           
Gel Documentation

Gel/Blot analysis


Nonlinear calibrations in X and Y dimensions.
1D mode.
1D mode for TLC.
Corrections for bent or stretched lanes, smiling.
Vertical, horizontal, rotatable, and user-traced lane definition tools.
Display and analyze multiple lanes, simultaneously, or one at a time. Manual or automatic peak detection. Manual or automatic baseline correction.

Powerful profile tools
Vertical, horizontal, rotatable, and user-traced lane definition tools. Display and analyze multiple profiles, simultaneously, or one at a time. Zoom in on any portion of the profile for high-resolution display.


           

Automated target detection


For grain counts, and other feature distribution analyses, fully automated target detection is supported. Multiple density thresholds can be combined with logical criteria (e.g. area) to separate targets from background.

Data report option

           
Gel Documentation

Sampling tools

 

Sampling tools are probes used to gather data from images. Tools can be sized and rotated by the user. Intersects can be drawn to divert sample lines away from structures and when combined with our Autoscan Sample tools it really is as easy as 1,2 click!

           
 

Sample Tools

  • rectangle
  • circle
  • trace
  • automatic
  • template
  • paint
  • polygon
  • whole field
  • distance
  • angle
  • pixel report
  • arrays

 

Theme colors

 

Skin Layout

  • Opened
  • Border
  • Boxed

Texture