MCID Analysis™ : Details

Details

MCID™ Analysis offers a powerful suite of image analysis tools and applications for the offline analysis of images, many of the analysis features of MCID™ Core are made available but with the acquisition, capture and hardware functionality removed. Also available in Server edition MCID™ Analysis is perfect to compliment existing MCID™ or other imaging system solutions.

Applications available include:

Autoradiography, Receptor Binding, Glucose Untilization & Regional Blood Flow
Grain & Cell Counting
Fluorescence Microscopy
Colocalisation
Gel / Blot Analysis
Stereology - (without Optical Fractionator)

MCID Analysis

Introduction

Details & Screenshots

FAQ

Application Guides
MCID Brochure (PDF)

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Display
MCID™ uses a single monitor, and displays the imported image and the analysis data in the same window. Therefore, we recommend a high resolution display, typically 1600x1200 pixels x 24 bits (16 million colors). MCID™ Analysis can also work with dual screens using Windows XP dual monitor support. For dual monitor support we recommend using an NVIDIA graphics card with at least 256MB RAM running at a resolution of 1600x1200 per screen.

Multiple Image Channels
MCID™ offers multiple, independent image channels (16 in total). Each channel can contain a discrete, calibrated image. Use the multiple image channels to align stained sections and autoradiographs, or to display images acquired at different fluorescence wavelengths. Each channel can have varying size images and are not limited by image size.

Image input / saving functions
MCID™ accepts and exports images in a variety of formats, including monochrome and color TIFF, MCID™, and in the native formats of many cameras and scanners. For other file formats, a general-purpose image format editor is supplied as standard. You can easily create your own image file format specifications, and integrate them within MCID™.

Calibration
Powerful calibration options allow you to calibrate images to external density and spatial standards. Nonlinear calibrations (e.g. molecular weight) fully supported.

Create calibration curves with best fit curve type options, special calibration functions for Glucose Untilisation, Protein Synthesis & Regional Blood Flow.

Data gathering & export
Powerful and flexible set of data sampling tools, including automatic tools. Data is saved to an MCID™ log file which contains scan shapes information and subject, section and region labelling, data can then be exported via the clipboard or in Excel, CSV, ASCII & Lotus formats.

Powerful Image Functions
Manipulate contrast, pseudocolor coding, nonlinear image mapping.

Image fusion - Collect any number of discrete fluor images. Fusion functions create 24-bit color images. Functions range from simple addition to weighted addition with layer-specific transparency.

Image processing and editing - Apply filters, logical operators, image arithmetic. Edit images to remove artifacts and much more.

Deconvolution - MCID™ includes both No-neighbour and Nearest-neighbour deconvolution algorithms.

Image Registration - Perform image registration on saved images.

Easy report generation
Report generator creates summary reports including numerical data, profile graphs, and image.

Print reports with one command.

Gel/Blot analysis
Nonlinear calibrations in X and Y dimensions.
1D mode.
1D mode for TLC.
Corrections for bent or stretched lanes, smiling.
Vertical, horizontal, rotatable, and user-traced lane definition tools.
Display and analyze multiple lanes, simultaneously, or one at a time. Manual or automatic peak detection. Manual or automatic baseline correction.

Powerful profile tools
Vertical, horizontal, rotatable, and user-traced lane definition tools. Display and analyze multiple profiles, simultaneously, or one at a time. Zoom in on any portion of the profile for high-resolution display.

Fully automatic target detection
For grain counts, and other feature distribution analyses, fully automated target detection is supported. Multiple density thresholds can be combined with logical criteria (e.g. area) to separate targets from background.

Sampling tools
Sampling tools are probes used to gather data from images. Tools can be sized and rotated by the user.

Sample Tools

rectangle
circle
trace
automatic

template
paint
polygon
whole field

distance
angle
pixel report
arrays

MCID™ Offers a powerful multipurpose image suite that would be a welcomed edition to any facility. That's why MCID™ has over 3000+ users worldwide from many of the worlds leading Pharmaceutical Companies, Universities and Research Facilities.